UElandy Plasmid Miniprep Kit, High-Purity Plasmid DNA Extraction, 50 Preps
UElandy Plasmid Miniprep Kit, High-Purity Plasmid DNA Extraction, 50 Preps
UElandy Plasmid Miniprep Kit, High-Purity Plasmid DNA Extraction, 50 Preps
UElandy Plasmid Miniprep Kit, High-Purity Plasmid DNA Extraction, 50 Preps
UElandy Plasmid Miniprep Kit, High-Purity Plasmid DNA Extraction, 50 Preps
UElandy Plasmid Miniprep Kit, High-Purity Plasmid DNA Extraction, 50 Preps
UElandy Plasmid Miniprep Kit, High-Purity Plasmid DNA Extraction, 50 Preps
UElandy Plasmid Miniprep Kit, High-Purity Plasmid DNA Extraction, 50 Preps

UElandy Plasmid Miniprep Kit, High-Purity Plasmid DNA Extraction, 50 Preps

$100.00
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UE-MN-P-50

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UElandy Plasmid Miniprep Kit, High-Purity Plasmid DNA Extraction, 50 Preps

UE Plasmid Miniprep Kit

The UE Plasmid Miniprep Kit utilizes an improved SDS alkaline lysis method combined with a silica-based DNA preparation membrane for rapid and efficient plasmid DNA purification. The system enables extraction of up to 30 μg of high-purity plasmid DNA from 1–4 mL bacterial cultures with OD600 values between 1.0 and 1.5.

Purified plasmid DNA is suitable for downstream molecular biology applications including DNA sequencing, in vitro transcription and translation, restriction enzyme digestion, bacterial transformation, cloning, and routine plasmid analysis workflows.

Improved SDS Alkaline Lysis Method

Optimized alkaline lysis chemistry supports rapid bacterial cell disruption and efficient plasmid DNA recovery.

Selective Silica Membrane Purification

DNA preparation membrane selectively binds plasmid DNA while removing contaminants and impurities.

High Purity Plasmid Recovery

Produces purified plasmid DNA suitable for sensitive downstream molecular biology applications.

Up to 30 μg DNA Yield

Supports extraction of up to 30 μg plasmid DNA from standard bacterial culture volumes.

Wide Molecular Biology Compatibility

Purified DNA is compatible with sequencing, restriction digestion, bacterial transformation, and transcription workflows.

Fast and Convenient Workflow

Streamlined protocol supports rapid plasmid purification for routine laboratory applications.

Technical Specifications

Property Value
Product Type Plasmid DNA Purification Kit
Purification Method Improved SDS alkaline lysis with silica membrane purification
Sample Type Bacterial culture
Recommended Culture Volume 1 – 4 mL
Recommended OD600 1.0 – 1.5
Maximum DNA Yield Up to 30 μg plasmid DNA
Maximum Plasmid Size Supported Up to 50 kb
Column DNA Binding Capacity Up to 20 μg DNA
Compatible Applications Sequencing, restriction digestion, transformation, transcription, translation
Elution Buffer Composition 2.5 mM Tris-HCl, pH 8.0

Kit Components

Component UE-MN-P-50
Kit Size 50 preps
Miniprep Columns 50
2 mL Microfuge Tubes 50
1.5 mL Microfuge Tubes 50
Buffer A 28 mL
RNase A 30 μL
Buffer S1 15 mL
Buffer S2 15 mL
Buffer S3 21 mL
Buffer W1 28 mL
Buffer W2 Concentrate 24 mL
Eluent 5 mL
Protocol Manual 1

Typical Applications

  • Plasmid DNA purification
  • DNA sequencing preparation
  • Restriction enzyme digestion
  • Bacterial transformation workflows
  • In vitro transcription and translation
  • Molecular cloning applications
  • Routine molecular biology research

Frequently Asked Questions

Why do some cultures produce different plasmid yields?

Plasmid yield is strongly influenced by plasmid copy number. Low-copy-number plasmids may require larger bacterial culture volumes.

Can this kit be used for large plasmids such as BACs?

This kit is not recommended for BAC extraction. Specialized large plasmid extraction kits are recommended for those applications.

Why is my plasmid yield low?

Low yield may result from excessive bacterial cells, incomplete lysis, failure to add ethanol to Buffer W2, or use of old bacterial cultures.

Why is there genomic DNA contamination?

Vigorous mixing during lysis or neutralization can cause genomic DNA contamination. Gentle handling during Buffer S2 and Buffer S3 steps is recommended.

Will the Eluent Buffer affect DNA sequencing?

No. The Eluent Buffer contains 2.5 mM Tris-HCl at pH 8.0 and does not interfere with DNA sequencing performance.

Storage & Stability

  • RNase A should be stored at -20°C for long-term storage.
  • Buffer S1 with RNase A added should be stored at 4°C.
  • All other buffers should be tightly closed and stored at room temperature.
  • Before use, add absolute ethanol to Buffer W2 concentrate according to label instructions.
  • If Buffer S2 forms precipitates, warm at 37°C until dissolved before use.

Quality & Compliance

Products manufactured in ISO-certified facilities under internationally recognized quality management systems for dependable molecular biology performance.

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