The PCR clean-up kit uses the method of selective adsorption of DNA preparation tubes to efficiently select and recover DNA fragments. It is suitable for the recovery of DNA from PCR or other enzymatic reactions with fragment lengths greater than 75 bp with a recovery rate of 70-90%. Up to 8 μg of linear DNA can be obtained per tube. There is no need to remove the mineral oil layer from the PCR reaction prior to purification.
The kit effectively removes primers, enzyme proteins, single nucleotides, fluorescent dyes, or radioisotopically labeled single nucleotides less than 50 mer. Purified DNA is suitable for a variety of uses, such as sequencing, ligation, restriction analysis, in vitro transcription, and microinjection.
Product Features
- Rapid centrifugation or negative pressure method
- Efficient purification of 75 bp-10 Kb DNA fragments
- Up to 8ug of linear DNA can be obtained
- Effectively remove primers less than 50 mer
- Keep the DNA fragments intact
- No ethanol precipitation is required
- Suitable for a wide range of downstream uses
- Three different packaging sizes
Experimental Results
Figure 1 Analysis of PCR products (1,3,5,7) and PCR products (2,4,6,8) after purification with the UElandy PCR Clean-up Kit.
Lanes 1, 2 - 5 Kb PCR products
Lanes 3, 4 - 1 Kb PCR products
Lanes 5, 6 – 500 bp PCR products
Lanes 7, 8 – 100 bp PCR products
1.5% agarose gel electrophoresis
Analyze the PCR products before purification and the PCR products after purification of the UElandy Cleanup Kit.
50% and 100% - 50% and 100% PCR products that are not purified, respectively. Lanes 1-6 - PCR product after purification. Each lane contained 5 Kb, 750 bp, 500 bp, and 100 bp DNA fragments, respectively. 1.5% agarose gel electrophoresis.
Flow Chart
