{"product_id":"uelandy-plasmid-miniprep-kit-high-purity-plasmid-dna-extraction-50-preps","title":"UElandy Plasmid Miniprep Kit, High-Purity Plasmid DNA Extraction, 50 Preps","description":"\u003cdiv class=\"product-info-section\"\u003e\n\u003csection class=\"product-summary\"\u003e\n\u003ch2\u003eUE Plasmid Miniprep Kit\u003c\/h2\u003e\n\u003cp\u003eThe UE Plasmid Miniprep Kit utilizes an improved SDS alkaline lysis method combined with a silica-based DNA preparation membrane for rapid and efficient plasmid DNA purification. The system enables extraction of up to 30 μg of high-purity plasmid DNA from 1–4 mL bacterial cultures with OD600 values between 1.0 and 1.5.\u003c\/p\u003e\n\u003cp\u003ePurified plasmid DNA is suitable for downstream molecular biology applications including DNA sequencing, in vitro transcription and translation, restriction enzyme digestion, bacterial transformation, cloning, and routine plasmid analysis workflows.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"feature-grid\"\u003e\n\u003cdiv class=\"feature-card\"\u003e\n\u003ch3\u003eImproved SDS Alkaline Lysis Method\u003c\/h3\u003e\n\u003cp\u003eOptimized alkaline lysis chemistry supports rapid bacterial cell disruption and efficient plasmid DNA recovery.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-card\"\u003e\n\u003ch3\u003eSelective Silica Membrane Purification\u003c\/h3\u003e\n\u003cp\u003eDNA preparation membrane selectively binds plasmid DNA while removing contaminants and impurities.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-card\"\u003e\n\u003ch3\u003eHigh Purity Plasmid Recovery\u003c\/h3\u003e\n\u003cp\u003eProduces purified plasmid DNA suitable for sensitive downstream molecular biology applications.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-card\"\u003e\n\u003ch3\u003eUp to 30 μg DNA Yield\u003c\/h3\u003e\n\u003cp\u003eSupports extraction of up to 30 μg plasmid DNA from standard bacterial culture volumes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-card\"\u003e\n\u003ch3\u003eWide Molecular Biology Compatibility\u003c\/h3\u003e\n\u003cp\u003ePurified DNA is compatible with sequencing, restriction digestion, bacterial transformation, and transcription workflows.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"feature-card\"\u003e\n\u003ch3\u003eFast and Convenient Workflow\u003c\/h3\u003e\n\u003cp\u003eStreamlined protocol supports rapid plasmid purification for routine laboratory applications.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"spec-section\"\u003e\n\u003ch2\u003eTechnical Specifications\u003c\/h2\u003e\n\u003ctable class=\"spec-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eProperty\u003c\/th\u003e\n\u003cth\u003eValue\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Type\u003c\/td\u003e\n\u003ctd\u003ePlasmid DNA Purification Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification Method\u003c\/td\u003e\n\u003ctd\u003eImproved SDS alkaline lysis with silica membrane purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Type\u003c\/td\u003e\n\u003ctd\u003eBacterial culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended Culture Volume\u003c\/td\u003e\n\u003ctd\u003e1 – 4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended OD600\u003c\/td\u003e\n\u003ctd\u003e1.0 – 1.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMaximum DNA Yield\u003c\/td\u003e\n\u003ctd\u003eUp to 30 μg plasmid DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMaximum Plasmid Size Supported\u003c\/td\u003e\n\u003ctd\u003eUp to 50 kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColumn DNA Binding Capacity\u003c\/td\u003e\n\u003ctd\u003eUp to 20 μg DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible Applications\u003c\/td\u003e\n\u003ctd\u003eSequencing, restriction digestion, transformation, transcription, translation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution Buffer Composition\u003c\/td\u003e\n\u003ctd\u003e2.5 mM Tris-HCl, pH 8.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003csection class=\"spec-section\"\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"spec-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eUE-MN-P-50\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKit Size\u003c\/td\u003e\n\u003ctd\u003e50 preps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMiniprep Columns\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2 mL Microfuge Tubes\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e1.5 mL Microfuge Tubes\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer A\u003c\/td\u003e\n\u003ctd\u003e28 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase A\u003c\/td\u003e\n\u003ctd\u003e30 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer S1\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer S2\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer S3\u003c\/td\u003e\n\u003ctd\u003e21 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer W1\u003c\/td\u003e\n\u003ctd\u003e28 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer W2 Concentrate\u003c\/td\u003e\n\u003ctd\u003e24 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEluent\u003c\/td\u003e\n\u003ctd\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProtocol Manual\u003c\/td\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003csection class=\"applications-section\"\u003e\n\u003ch2\u003eTypical Applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePlasmid DNA purification\u003c\/li\u003e\n\u003cli\u003eDNA sequencing preparation\u003c\/li\u003e\n\u003cli\u003eRestriction enzyme digestion\u003c\/li\u003e\n\u003cli\u003eBacterial transformation workflows\u003c\/li\u003e\n\u003cli\u003eIn vitro transcription and translation\u003c\/li\u003e\n\u003cli\u003eMolecular cloning applications\u003c\/li\u003e\n\u003cli\u003eRoutine molecular biology research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection class=\"faq-section\"\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003ch3\u003eWhy do some cultures produce different plasmid yields?\u003c\/h3\u003e\n\u003cp\u003ePlasmid yield is strongly influenced by plasmid copy number. Low-copy-number plasmids may require larger bacterial culture volumes.\u003c\/p\u003e\n\u003ch3\u003eCan this kit be used for large plasmids such as BACs?\u003c\/h3\u003e\n\u003cp\u003eThis kit is not recommended for BAC extraction. Specialized large plasmid extraction kits are recommended for those applications.\u003c\/p\u003e\n\u003ch3\u003eWhy is my plasmid yield low?\u003c\/h3\u003e\n\u003cp\u003eLow yield may result from excessive bacterial cells, incomplete lysis, failure to add ethanol to Buffer W2, or use of old bacterial cultures.\u003c\/p\u003e\n\u003ch3\u003eWhy is there genomic DNA contamination?\u003c\/h3\u003e\n\u003cp\u003eVigorous mixing during lysis or neutralization can cause genomic DNA contamination. Gentle handling during Buffer S2 and Buffer S3 steps is recommended.\u003c\/p\u003e\n\u003ch3\u003eWill the Eluent Buffer affect DNA sequencing?\u003c\/h3\u003e\n\u003cp\u003eNo. The Eluent Buffer contains 2.5 mM Tris-HCl at pH 8.0 and does not interfere with DNA sequencing performance.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"storage-section\"\u003e\n\u003ch2\u003eStorage \u0026amp; Stability\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRNase A should be stored at -20°C for long-term storage.\u003c\/li\u003e\n\u003cli\u003eBuffer S1 with RNase A added should be stored at 4°C.\u003c\/li\u003e\n\u003cli\u003eAll other buffers should be tightly closed and stored at room temperature.\u003c\/li\u003e\n\u003cli\u003eBefore use, add absolute ethanol to Buffer W2 concentrate according to label instructions.\u003c\/li\u003e\n\u003cli\u003eIf Buffer S2 forms precipitates, warm at 37°C until dissolved before use.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection class=\"quality-section\"\u003e\n\u003ch2\u003eQuality \u0026amp; Compliance\u003c\/h2\u003e\n\u003cp\u003eProducts manufactured in ISO-certified facilities under internationally recognized quality management systems for dependable molecular biology performance.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e","brand":"UElandy","offers":[{"title":"Default Title","offer_id":53615307817279,"sku":"UE-MN-P-50","price":100.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0843\/7687\/9423\/files\/UE-ND-EP-10.png?v=1739848633","url":"https:\/\/bioscience-usa.com\/products\/uelandy-plasmid-miniprep-kit-high-purity-plasmid-dna-extraction-50-preps","provider":"BioScience USA Inc","version":"1.0","type":"link"}