{"product_id":"uelandy-pcr-cleanup-kit-ue-pcr-250-recover-up-to-8-μg-of-linear-dna-from-pcr-or-other-enzymatic-reactions","title":"UElandy PCR Cleanup Kit UE- PCR-250, Recover up to 8 μg of linear DNA from PCR or other enzymatic reactions","description":"\u003cp class=\"p1\"\u003eThe PCR clean-up kit uses the method of selective adsorption of DNA preparation tubes to efficiently select and recover DNA fragments. It is suitable for the recovery of DNA from PCR or other enzymatic reactions with fragment lengths greater than 75 bp with a recovery rate of 70-90%. Up to 8 μg of linear DNA can be obtained per tube. There is no need to remove the mineral oil layer from the PCR reaction prior to purification.\u003c\/p\u003e\n\u003cp class=\"p1\"\u003eThe kit effectively removes primers, enzyme proteins, single nucleotides, fluorescent dyes, or radioisotopically labeled single nucleotides less than 50 mer. Purified DNA is suitable for a variety of uses, such as sequencing, ligation, restriction analysis, in vitro transcription, and microinjection.\u003c\/p\u003e\n\u003ch3 class=\"p1\"\u003e\u003cstrong\u003eProduct Features\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"p1\"\u003eRapid centrifugation or negative pressure method\u003c\/li\u003e\n\u003cli class=\"p1\"\u003eEfficient purification of 75 bp-10 Kb DNA fragments\u003c\/li\u003e\n\u003cli class=\"p1\"\u003eUp to 8ug of linear DNA can be obtained\u003c\/li\u003e\n\u003cli class=\"p1\"\u003eEffectively remove primers less than 50 mer\u003c\/li\u003e\n\u003cli class=\"p1\"\u003eKeep the DNA fragments intact\u003c\/li\u003e\n\u003cli class=\"p1\"\u003eNo ethanol precipitation is required\u003c\/li\u003e\n\u003cli class=\"p1\"\u003eSuitable for a wide range of downstream uses\u003c\/li\u003e\n\u003cli class=\"p1\"\u003eThree different packaging sizes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"p1\"\u003eExperimental Results\u003c\/h3\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0843\/7687\/9423\/files\/UElandy_PCR_Cleanup_Kit-1.png?v=1788466247\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp class=\"p1\"\u003e\u003cstrong\u003eFigure 1 Analysis of PCR products (1,3,5,7) and PCR products (2,4,6,8) after purification with the UElandy PCR Clean-up Kit.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"p1\"\u003eLanes 1, 2 - 5 Kb PCR products\u003c\/p\u003e\n\u003cp class=\"p1\"\u003eLanes 3, 4 - 1 Kb PCR products\u003c\/p\u003e\n\u003cp class=\"p1\"\u003eLanes 5, 6 – 500 bp PCR products\u003c\/p\u003e\n\u003cp class=\"p1\"\u003eLanes 7, 8 – 100 bp PCR products\u003c\/p\u003e\n\u003cp class=\"p1\"\u003e1.5% agarose gel electrophoresis\u003c\/p\u003e\n\u003cp class=\"p1\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0843\/7687\/9423\/files\/UElandy_PCR_Cleanup_Kit-2.png?v=1788466247\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp class=\"p1\"\u003e\u003cstrong\u003eAnalyze the PCR products before purification and the PCR products after purification of the UElandy Cleanup Kit.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"p1\"\u003e50% and 100% - 50% and 100% PCR products that are not purified, respectively. Lanes 1-6 - PCR product after purification. Each lane contained 5 Kb, 750 bp, 500 bp, and 100 bp DNA fragments, respectively. 1.5% agarose gel electrophoresis.\u003c\/p\u003e\n\u003ch3 class=\"p1\"\u003e\u003cstrong\u003eFlow Chart\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0843\/7687\/9423\/files\/UElandy_PCR_Cleanup_Kit-3.png?v=1788466247\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"UElandy","offers":[{"title":"UE-MX-PCR-10","offer_id":55375715107135,"sku":null,"price":24.9,"currency_code":"USD","in_stock":false},{"title":"UE-MX-PCR-50","offer_id":55375715139903,"sku":null,"price":79.0,"currency_code":"USD","in_stock":false},{"title":"UE-MX-PCR-250","offer_id":55375715172671,"sku":null,"price":249.0,"currency_code":"USD","in_stock":true},{"title":"UE-MX-PCR-500","offer_id":55375715205439,"sku":null,"price":449.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0843\/7687\/9423\/files\/UE-PCR-250_d6231b28-2279-46f7-b3d3-48fa78e5b925.png?v=1788470981","url":"https:\/\/bioscience-usa.com\/products\/uelandy-pcr-cleanup-kit-ue-pcr-250-recover-up-to-8-%ce%bcg-of-linear-dna-from-pcr-or-other-enzymatic-reactions","provider":"BioScience USA Inc","version":"1.0","type":"link"}